Thermo verso cdna synthesis kit
WebbVerso cDNA Synthesis Kit • GCリッチなテンプレートやその他の困難なテンプレートを使用して良好な結果を得るための広い動作温度範囲（42～57℃） • RTエンハンサーを内 … WebbThermo Scientific Verso cDNA synthesis Kit provides robust transcription of RNA to create a complete cDNA pool. The Verso system achieves robust and sensitive reverse... Shop. English English; Change Country. Request a Quote › Help & Support Center › …
Thermo verso cdna synthesis kit
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WebbThermo Scientific Verso cDNA Synthesis Kits by Thermo Fisher Scientific Manufacturer Thermo Fisher Scientific Be the first to review this product The Thermo Scientific Verso … Webbchemically modified hot-start version of Thermo Scientific ThermoPrime Taq DNA Polymerase, which prevents non-specific amplification during cDNA synthesis. Thermo-Start™ has 5' to 3' polymerization and exonuclease activity but lacks 3' to 5' exonuclease activity (proofreading). Thermo-Start requires an activation step at 95 °°°C for 15 ...
WebbThermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit uses RevertAid … Webb効率的にrnaの逆転写を行うための、高温でも反応活性が高いcdna合成キット cDNA合成キット Thermo Fisher Scientific - JP Thermo Fisher Scientific Logo
Webb10 aug. 2024 · At confluency, the cells were harvested and RNA was extracted using the RNeasy kit. To quantify mRNA expression, 1μg RNA per sample was reverse-transcribed to cDNA using the Verso cDNA synthesis kit (Thermo Fisher) according to manufacturer’s instructions using a 3:1 blend of random hexamers and anchored oligo-dT. Webb25 sep. 2015 · Verso cDNA synthesis kit Catalog Number: AB-1453/B This product was used to check the gene expression changes in glioblastoma cells in differential treatment conditions. The product is easy to use and less time consuming in synthesis of cDNA from mRNA. Experimental Design and Results Summary Application cDNA synthesis Starting …
WebbEl kit de síntesis de ADNc Verso incluye la mezcla de enzimas RT con inhibidor de ARNasa, tampón RT 5X, hexámeros aleatorios, oligo dT anclado, 20 mM de dNTP, RT Enhancer. Almacene a -20 °C. Método de PCR
WebbThermo Scientific RevertAid 第一链 cDNA 合成试剂盒（含 DNase I）是根据 RNA 模板高效合成第一链 cDNA 的完整系统。 该试剂盒含有重组核酸内切酶 (DNase I)，可从 RNA 制剂中去除污染的基因组 DNA。 针对逆转录应用，此试剂盒使用了 RevertAid 逆转录酶 (RT)，它是一种 M-MuLV Rt，可在 42 50°C 条件下保持活性且适合合成长达 13 kb 的 cDNA。 随试 … market place hillcrestWebbThermo Scientific Verso cDNA synthesis Kit provides robust transcription of RNA to create a complete cDNA pool. The Verso system achieves robust and sensitive reverse... Shop marketplace hk online shoppingWebbThermo Scientific Verso cDNA synthesis Kit provides robust transcription of RNA to create a complete cDNA pool. The Verso system achieves robust and sensitive reverse … market place high point ncWebbAB-1453/B Verso cDNA Kit Packaging ... 00991644 0.1 mL Random Hexamers 00978636 0.1 mL Anchored Oligo-dT 00969757 0.5 mL 5x cDNA Synthesis Buffer QUALITY CONTROL Parameter Method Requirement Result Functional testing Verso cDNA Kit is tested functionally for use in RT-PCR. Conforms Conforms ISO CERTIFICATION Manufactured … navigational directionWebbThe SuperScript™ III First-Strand Synthesis System for RT-PCR is optimized to synthesize first-strand cDNA from purified poly(A) + or total RNA. RNA targets from 100 bp to >12 kb can be detected with this … marketplace hmsWebb16 aug. 2024 · cDNA synthesis steps There are different steps for synthesis of cdna such as; Prepare sample Remove genomic DNA Select reverse transcriptase Prepare reaction mix Perform cDNA synthesis cDNA synthesis protocol 1. Prepare sample The RNA template is used for cDNA production. market place hitchin postcodeWebb15 maj 2013 · To this, 9 µl of master mix* was added and cDNA was synthesized at 42°C for 1 hour followed by 92°C for 2 mins. Finally, cDNA was stored at -20 °C until further use. (*20 µL master mix = 4 µL 5X cDNA synthesis buffer + 2 µL 1X dNTP Mix + 1 µL Random Hexamer + 1 µL RT Enhancer + 1 µL Verso Enzyme Mix) navigational ease