2024 Svct2 western blot

Svct2 western blot

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August undefined, 2024

Splet蛋白免疫印迹（Western Blot）是将电泳分离后的细胞或组织中蛋白质从凝胶转移到固相支持物NC膜或 PVDF膜上，然后用特异性抗体检测某特定抗原的一种蛋白质检测技术， … SpletBackground and objective. Activation and abnormal expression of histone deacetylase (HDAC) which is important target for cancer therapeutics are related to the occurrence of human leukemia. 20(s)-Ginsenoside Rh2 (20(s)-Rh2) may be a potential HDAC inhibitor (HDACi) of leukemia, but the mechanism has not been reported.

Sodium-Dependent Vitamin C Transporter 2 Deficiency Causes ...

Splet02. jan. 2024 · Il principio del western blotting (o Immunoblotting o Immunofissazione) si basa sull’identificazione, con un anticorpo specifico, di un determinato antigene (proteina) presente all’interno di una complessa miscela di antigeni (o proteine) separate in un gel di poliacrilammide in base al peso molecolare e immobilizzate su una membrana. Splet(c) Western blot analysis of U-U937 and D-U937 cell-derived mitochondrial lysates (10 and 15 μ g of mitochondrial proteins) using anti-SVCT2 antibodies. Anti-HSP-60 antibodies were used to provide an internal loading control and for comparative densitometric analysis. iowa st women\u0027s basketball

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Splet01. avg. 2024 · Analysis of SVCT2 expression by RT-PCR and Western blot in four breast cancer cell lines are shown (Fig. 1). Immunofluorescence colocalization assays for … Splet23. nov. 2011 · Two days after transfection, Schwann cells were harvested for Western blotting or real-time PCR. SVCT2 siRNA-treated Schwann cell cultures showed a significant reduction in collagen V chains (Fig. 7B). Western blot bands of SVCT2 showed weaker bands in siRNA-treated Schwann cell cultures, confirming successful knockdown (Fig. 7B). Splet11. feb. 2011 · Time-course analyses of SVCT2 expression by immunohistochemistry and western blots showed upregulation in the subacute phase of 2-5 days. Radioactive uptake assays using (14)C-labelled ascorbic acid showed a significant increase of ascorbic acid uptake into the brain after stroke. open h\u0026r block tax software

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SpletSVCT2 staining (green) in CA1 hippocampal region is detected in axonal processes (arrows), with the highest density surrounding the pyramidal layer (P). Cell nuclei are stained... View more Product Details Product Specific Information Reconstitution: 1 X 50 µL double distilled water (DDW), depending on the sample size. SpletTecnólogo Médico en Morfofisiopatología y Citodiagnóstico, además cursé 4 años de Doctorado en Ciencias Biológicas, área Biología Celular y Molecular. Experiencia en diversas técnicas de laboratorio: PCR, Inmunohistoquímica, Inmunofluorescencia, Western Blot, Histología, Cultivo celular primario y de líneas celulares, Microscopía, entre otras. … open http port windows 10SpletWestern blot. The tissues extracted from motor cortices and ischemic penumbra areas at different time points were lysed in precooled RAPI buffer. Total proteins were separated by 10% SDS-PAGE gels; then, the protein in gels was transferred onto PVDF membranes. After blockage in 3% bovine serum albumin for 2 h, the membranes were incubated with ... iowa st wrestling

"Splet24. jun. 2016 · PCR for SVCT1 and SVCT2 was performed 40 cycles of denaturation at 95℃ for 45 seconds, annealing at 55℃ or 61℃ respectively for 45 seconds, and amplification at 72℃ at 45 seconds. ... To figure out this point, we performed reverse transcription polymerase chain reaction (RT-PCR) and western blotting for these molecules in T cells. … " - Svct2 western blot

Svct2 western blot

SpletThis gene encodes one of the two required transporters and the encoded protein accounts for tissue-specific uptake of vitamin C. Previously, this gene had an official symbol of … Splet06. maj 2024 · In this article, we examined the SVCT2 transporter expression in various breast cancer cell lines using RT-PCR and Western blot assays. In addition, we analyzed …

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Splet13. avg. 2013 · (L) Western blot analysis for βIII-tubulin and SVCT2 expression in neurospheres cultured in the presence of AA or control medium. (M,N) Quantitative analysis of the Western blot. The data represent the average and standard deviation of three experiments analyzed by One-Way ANOVA with the Bonferroni post-test. SpletWestern Blot: Human: Lee SH, Kim JS, Jun HK The major outer membrane protein of a periodontopathogen induces IFN-beta and IFN-stimulated genes in monocytes via lipid raft and TANK-binding kinase 1/IFN regulatory factor-3. J. Immunol., 2009;182(9):5823-35. 2009 [PMID: 19380831] (Western Blot, Human)

SpletSLC23A2 Antibody. NBP2-13319. Species: Hu, Mu, Rt. Applications: WB, ICC/IF. Publications using NBP2-13319. Applications. Species. Cho S, Chae JS, Shin H et al. Hormetic dose … Splet04. dec. 2024 · Anti-SVCT2/SLC23A2 (extracellular) Antibody (#AST-022) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize SVCT2 from rat, mouse, and human samples.

Splet1. Western blotting 원리. Western blot은 세포나 조직에서 얻은 단백질들에서 특정 단백질 (specific protein)을 검출할 수 있는 실험 방법이다. 기본 원리는 단백질을 size별로 분류한 뒤에 발현 양을 확인하고 싶은 항체를 이용하여 확인 하는 것이다. 즉, 항원-항체 반응을 ... SpletEnter the email address you signed up with and we'll email you a reset link.

SpletAnti-SVCT2 Western Blot Antibody Products. Products (26) Write a Review; Company View; Product View; Your search returned 26 SVCT2 Western Blot Antibodies across 5 suppliers. Showing 5 of 5 suppliers (26 products total) > >> Select All. Select up to 5 products from below to compare or request more information.

SpletSVCT2 is a reported alias name for the human gene SLC23A2, or 'solute carrier family 23 member 2'. The 650-amino acid protein is a member of the Xanthine/uracil permease family, Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily. The cellular localization is predicted to be membrane-associated. Glycosylation sites have been reported. iowa st women\u0027s basketball scheduleSpletThe concentrations of glutamate and ascorbate in brain extracellular fluid increase following seizure activity, trauma and ischemia. Extracellular ascorbate concentration also rises following intracerebral glutamate injection. We hypothesized that glutamate triggers the release of ascorbate from astrocytes. iowa st wrestling scheduleSpletSVCT2 antibody LS-C334917 is an unconjugated rabbit polyclonal antibody to SVCT2 (SLC23A2) from human. It is reactive with human, mouse and rat. Validated for WB. … open hub destination in sap bwSplet03. feb. 2012 · The western (note that in this context “western” should be spelt with a lower-case “w”) blot is commonly used to identify, quantify, and determine the size of specific proteins. Western blotting evolved from Southern blotting, which is used to detect specific DNA sequences among DNA fragments separated by gel electrophoresis, and ... open http proxy listSplet08. dec. 2015 · Western blot analysis of SVCT2 overexpression using anti-GFP antibodies in total protein extracts from non-transfected and EYFP- or hSVCT2wt-EYFP-overexpressing cells ( h ). Immunofluorescence analysis with an anti-MAP2 antibody (Cy3, red channel) in hSVCT2wt-EYFP-overexpressing cells ( green channel ). open h\u0026r block softwareSpletAlomone Labs, LTD Anti-SVCT2/SLC23A2 (extracellular) Polyclonal, Catalog # AST-022-200UL. Tested in Western Blot (WB), Immunohistochemistry (Frozen) (IHC (F)) and Flow … open huawei lock screen with kali linuxSpletO western blot (por vezes chamado imunoblot de proteínas ou transferência western) é um método em biologia molecular e bioquímica para detectar proteínas numa amostra de homogenato (células bem trituradas) de tecidos biológicos ou estratos. Essa técnica usa eletroforese em gel para separar as proteínas nativas pela sua estrutura tridimensional … open https site in file explorer