2024 Scycf1

Scycf1

Author: jogd

August undefined, 2024

WebbPCR dengan menggunakan primer untuk ekson1-ekson2 dari gen aktin menghasilkan DNA yang berukuran sekitar 450 pb (Gambar 2) yang menunjukkan bahwa daerah yang diamplifikasi adalah WebbScYCF1 is an ABC trans-porter of Saccharomyces cerevisae that contributes to Cd(II) resistance by pumping glutathione-conjugated cadmium(II) into the vacuole (Li et al., 1997), and SpHMT1, a half-size ABC transporter from Schizosaccharomyces pombe , contributes to

Gene amplification PCR product. M: DNA Marker DL5000; 1-2: …

Webb14 apr. 2024 · Do not Store this antibody in 4°C. tested applications: Immunofluorescence (IF), Immunolocalization (IL), Western blot (WB) background: The Plasma Membrane … cocktail ironing board

Cloning and Ectopic Expression of ScYCF1 Gene from …

WebbScYCF1 is alkaline and positive charged, stable, and hydrophilic protein. The predictive secondary structure is mainly composed of α-helix areas, random coils and β-sheets. We … WebbScYCF1 is alkaline and positive charged, stable, and hydrophilic protein. The predictive secondary structure is mainly composed of α-helix areas, random coils and β-sheets. We … WebbDevelopment of transgenic rice with OsABCC1, c-ECS Reduced As translocation from root to the grains in and ScYCF1 RCc3p-AEY plants To reduce As accumulation in rice grains, we developed trans- All lines exhibited reduced As accumulation in grains and flag genic rice expressing two different vacuolar As sequestration leaves compared to the WT and … cocktail irish car bomb

Anti-H+ATPase (plasma membrane) (Global antibody) - Agrisera

An ATP‐binding cassette transporter related to yeast vacuolar …

WebbDownload scientific diagram Gene amplification PCR product. M: DNA Marker DL5000; 1-2: ScYCF1 gene. from publication: Cloning and Ectopic Expression of ScYCF 1 Gene … Webb13 feb. 2006 · In this review, we will give a detailed overview of the mutation analysis reported for selected ABC transporters of the ABCB and ABCC subfamilies, namely HsCFTR/ABCC7, HsSUR/ABCC8,9, HsMRP1/ABCC1, HsMRP2/ABCC2, ScYCF1 and P-glycoprotein (Pgp)/MDR1/ABCB1 and their effects on the function of each protein. callout verification failureWebbComputer searches detected the significant resemblance of CrMRP2 with HsMRP1, AtMRP3 and ScYCF1, in Homo sapiens, Arabidopsis thaliana and Saccharomyces cerevisiae, respectively. All are members of the multidrug resistance-associated protein (MRP)/cystic fibrosis transmembrane conductance regulator (CFTR) subfamily of ATP … cocktail invitations templates free

"WebbThe complementation of the Scycf1 strain was found BSO-sensitive, suggesting that cadmium conjugated to glutathione is a substrate of SpHMT1. Overexpression of a … " - Scycf1

Scycf1

An ATP-binding cassette transporter related to yeast vacuolar …

Webb43 ∆scycf1 yeast mutant (Tommasini, Vogt, Fromenteau, Hortensteiner, Matile, Amrhein & 44 Martinoia 1998) and conversely the overexpression of ScYCF1 in A. thaliana enhances the 45 tolerance of the plant to Pb 2+ and Cd 2+ (Song, Ju Sohn, Martinoia, Jik Lee, Yang, Jasinski, 46 Forestier, Hwang & Lee 2003). WebbThe ScYCF1 protein of the yeast Saccharomyces cerevi-sae, a full-size ABC transporter of the MRP (‘multidrug-resistance-related protein’) subfamily, ensures the transport of …

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Webb1 okt. 2006 · An ATP-binding cassette transporter related to yeast vacuolar ScYCF1 is important for Cd sequestration in Chlamydomonas reinhardtii. 1 Europe PMC requires … Webb20 juli 2006 · ScYCF1 was identified as a vacuolar GSH S‐conjugate pump that conferred Cd resistance in S. cerevisiae (Li et al. 1996). HsMRP1 and AtMRP3 were also …

Webb28 jan. 2024 · By contrast, three phosphorylation sites were detected in ScYCF1 [17, 18] and 10 in HsABCC7, also known as CFTR . However, the absence of altered phenotypes by single amino acid mutation does not exclude the possibility that these residues are involved in phosphorylation-mediated regulation. WebbScYcf1. dari ragi dan . HmMrp1. dari manusia. Komposisi PCR untuk . MaMrp. sama dengan untuk aktin, hanya primernya yang diganti dengan . mrpF. dan . mrpR. Kondisi PCR untuk . MaMrp. adalah sama dengan untuk aktin. Pengklonan fragmen cDNA . MaMrp. Fragmen cDNA . MaMrp. diligasikan dengan pGEM-T Easy (Promega Inc.) dengan …

WebbScYCF1 is alkaline and positive charged, stable, and hydrophilic protein. The predictive secondary structure is mainly composed of α-helix areas, random coils and β-sheets. We … Webbcerevisiae (ScYCF1) in the cotton, ScYCF1 was cloned from Saccharomyces cerevisiae As2.375, with the full-length of 4548 bp. The bioinformatics analy-sis revealed that the …

WebbHinder et al. (1996) On the basis of the functional resemblance of plant vacuolar suggest that the transport function responsible for Mg-ATP– GS conjugate transport to that mediated by ScYCF1 and energized uptake of the chlorophyll catabolite Bn-NCC-1 HmMRP1 and the 44.6% sequence identity (63.9% similarity) into isolated vacuoles from …

WebbWe developed transgenic rice plants expressing two different vacuolar As sequestration genes, ScYCF1 and OsABCC1, under the control of the RCc3 promoter in the root cortical … callout vectorWebb1 juli 1997 · On the assumption that the complete ORF of the gene corresponding to this cDNA was similar in size to those of ScYCF1 and HmMRP1, it was estimated to be missing a minimum of 1.5 kb of 5′ coding sequence. Hence, to isolate clones containing this missing sequence, 500 bp of the 5′ terminus of the 3.5-kb cDNA was used to screen two ... cocktail irishWebbIn Saccharomyces cerevisiae, ScYCF1 represents a vacuolar membrane protein involved in heavy metal and drug detoxiﬁ-cation. Disruption of the gene results in a yeast strain hyper-sensitive to cadmium [37]. ScYCF1 is an ATP-dependent pump able to transport organic glutathione-S-conjugates (GS-conjugates) into the vacuole [38], GS–metal ... cocktail is cared aboutWebb1 nov. 2006 · Request PDF An ATP-binding cassette transporter related to yeast vacuolar ScYCF1 is important for Cd sequestration in Chlamydomonas reinhardtii We generated … call out vet near meWebb1 feb. 1998 · On the basis of the functional resemblance of plant vacuolar GS conjugate transport to that mediated by ScYCF1 and HmMRP1 and the 44.6% sequence identity (63.9% similarity) between ScYCF1 and … callout viewWebb1 juni 1998 · Abstract While the concept of H + -coupling has dominated studies of energy-dependent organic solute transport in plants for over two decades, recent studies have demonstrated the existence of a group of organic solute transporters, belonging to the ATP-binding cassette (ABC) superfamily, that are directly energized by MgATP rather … callout vs callbackWebbThe chlorophyll content of the leaf discs processed was measured and results showed that under three kinds of treatment, the chlorophyll content of leaf discs in transgenic plants was cocktail islay scotch