2024 Puc19 control vector linearized 50 ng/μl amp+

Puc19 control vector linearized 50 ng/μl amp+

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August undefined, 2024

WebA “stuffer” sequence allows for electrophoretic separation of linearized vector from the reaction ... 10-50 ng/μl: 1: dGTP (100mM) 2.5 mM: 2: DTT (100 mM) 5 mM: 1: BSA (10 … WebAug 4, 2013 · 5X In-Fusion HD Enzyme Premix 20 μl 100 μl 200 μl. pUC19 Control Vector, linearized (50 ng/μl) 5 μl 15 μl 30 μl. 2 kb Control Insert (40 ng/μl) 10 μl 30 μl 60 μl. III. …

In-Fusion HD Cloning Kit User Manual - ResearchGate

Web100 μl dNTP Mix 12.5 mM each dATP, dCTP, dGTP, and dTTP; neutralized at pH 8.0 in water 10 μl Salt Solution 1.2 M NaCl 0.06 M MgCl 2 50 μl Sterile Water – 1 ml Control PCR template 50 ng/μl in TE buffer*, pH 8.0 10 μl Control PCR primers 100 ng/μl each in TE buffer, pH 8.0 10 μl Polyhedrin forward sequencing primer 100 ng/μl in TE ... WebLinearized version of pUC19 for use with the GeneArt® Seamless Cloning and Assembly Kit. ... ApoI is typically used at 50°C, but is 50% active at 37°C. EcoRI (2643) 1 site: G A A T T C … gas company turned off gas

lab1.pdf - 22 There exist a great variety of plasmids that...

WebLinearized pMiniT ™ 2.0 Vector 20 µl 20 µl Amplicon Cloning Control (1 kb) 10 µl 10 µl ... µl 35 µl Cloning Analysis Reverse Primer 35 µl 35 µl NEB 10-beta Competent E. coli (Cloning Efficiency) 20 x 50 µl pUC19 Control DNA 25 µl NEB 10 -beta/Stable Outgrowth Medium 25 ml *Cloning mixes 1 and 2 provide T4 DNA Ligase, ... WebAug 24, 2024 · It is a commonly used cloning vector in the bacteria E. coli . pUC19 is 2686 bp in length. The molecular weight of the pUC19 vector is 1.75×10 6 Da. It is a small … WebAll In-Fusion HD Cloning kits contain 5X In-Fusion HD Enzyme Premix, linearized pUC19 Control Vector (50 ng/μl), and 2 kb Control Insert (40 ng/μl). Store all components at … gas company vancouver

NEB PCR Cloning Kit (with or without competent cells) E1202 …

Web2×TOOLSwift Assembly Mix (25 ng/µl) 2x50 µl pUC19 Control Vector, linearized (50 ng/μl) (5 u/µl) 10 µl 2 kb Control Blunt Insert (50 ng/μl) 10 µl ddH 2 O 2x1 ml Storage All … WebVector Map Clontech Laboratories, Inc. A Takara Bio Company 1290 Terra Bella Avenue, Mountain View, CA 94043, USA U.S. Technical Support: [email protected] United … gas company venturaWebneeds. The number of moles of pMD19-T Vector 1 μl (50 ng) is approximately 0.03 pmol. Control Insert Control Insert is a 500 bp PCR product with an A base at the 3'end. The … gas company vero beach

"Web489 .. 505 = 17 bp. The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D … " - Puc19 control vector linearized 50 ng/μl amp+

Puc19 control vector linearized 50 ng/μl amp+

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WebThis kit takes advantage of proprietary recombinases and homologous recombination to achieve directional recombination of PCR inserts with 15-25bp overlapping ends into any … Web50 μl: 100 μl: 3×100 μl: Linearized pUC19 Control Vector (10 ng/μl) 3 μl: 6 μl: 18 μl: Control Insert (1 kb，20 ng/μl） 3 μl: 6 μl: 18 μl: Trans1-T1 Phage Resistant Chemically …

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WebResults RNase P assay Plasmid pUC19 was modified into an in vitro transcription system by incorporation of T7 promoter sequence and Reconstituted E. coli RNase P, a ptRNA … WebB expression clone) and the donor vector • 50 femtomoles (fmol) each. of . att. B PCR product (or linearized . att. B expression clone) and donor vector is preferred, but the …

http://www.jetfabio.com/pic/VIT-BB01_TOOLSwift_PCR_Cloning_Kit.pdf Web500 bp control insert (20 ng/μl) 5 μl. 5 μl. pUC19 control vector，linearized (50 ng/μl，Amp+ ...

WebThe digested pUC19 samples were purified using a phenol extraction of DNA sample and ethanol precipitation of DNA (1). pUC19 was then dissolved in dH2O and stored at -20ºC. Two hundred and fifty microgram of digested, purified pUC19 DNA was ligated in a 20 μL reaction mix of 1 μL T4 DNA ligase, 1X Ligation buffer, and dH2O for 16 hours 4, 16 ...

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WebInclude an extra tube for control DNA, if desired; Allow the cells to thaw for 5 minutes. Gently tap the tubes multiple times to obtain uniform suspension. For control: Add 1 µL (10 ng) … gas company victorvilleWebpENTR™/U6 vector, linearized 0.5 ng/μL in: 10 mM Tris-HCl, pH 8.0 1 mM EDTA, pH 8.0 ... pUC19 Control DNA 10 pg/μL in 5 mM Tris-HCl, 0.5 mM EDTA, pH 8 50 μl Genotype of … gas company vermontWeb10 μl 50 μl 100 μl 5X In-Fusion Reaction Buffer 20 μl 100 μl 200 μl pUC19 Control Vector,* linearized (50 ng/μl) 5 μl 5 μl 5 μl 2 kb Control Insert (40 ng/μl) 10 μl 10 μl 10 μl … gas company tucson azWebFirstly, the vector is linearized at the cloning site. A small sequence overlapped with each end of the cloning site is added onto the insert through PCR. The insert and the linearized vector, with overlapped sequences of 15 bp - 20 bp on both 5' - and 3' -end, respectively, are mixed in an appropriate ratio and incubated with recombinase at 50℃ for 5-15 min. david and goliath snackWebSalt Solution 1.2 M NaCl, 0.06 M MgCl2 50 μL Sterile Water — 1 mL Control PCR template 50 ng/μL in TE buffer[1], pH 8.0 10 μL Control PCR primers 100 ng/μL each in TE buffer, pH 8.0 10 μL Polyhedrin forward sequencing primer 100 ng/μL in TE buffer, pH 8.0 20 μL SV40 polyA reverse sequencing primer 100 ng/μL in TE buffer, pH 8.0 20 μL gas company victoriaWebThe digested pUC19 samples were purified using a phenol extraction of DNA sample and ethanol precipitation of DNA (1). pUC19 was then dissolved in dH2O and stored at -20ºC. … gas company van nuysWebOne Step Express Cloning Kit provides 5 μl of pUC19 control vector (linearized, 50 ng/μl) and 5 μl of control insert (0.5 kb, 25 ng/ μl). Use 1 μl of each for a positive control … david and goliath stone craft