Promega bactiter glo
WebNov 1, 2024 · Our procedure involved using a modified broth dilution method that screened compounds in 96-well broth cultures of H. pylori using the commercially available BacTiter-Glo Microbial Cell Viability Assay (Promega) to determine the half-maximal effective concentration (EC [sub.50]) of each compound. WebBacTiter-Glo™ Microbial Cell Viability Assay INSTRUCTIONS FOR USE OF PRODUCTS G8230, G8231, G8232 AND G8233. PROTOCOL Quick Before beginning the protocol, see Section 3.A., Reagent Preparation, in the BacTiter-Glo™ Microbial Cell Viability Assay Technical Bulletin , #TB337. 1. Perform the following steps at room temperature …
Promega bactiter glo
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WebAug 16, 2016 · 双萤光素酶基因技术详解.ppt,Report Smartly －萤光素酶报告基因技术 自然界的萤光 自然界的萤光 报告基因的作用 细胞信号转导途径 启动子/增强子 受体结合 病毒/细胞相互作用 转录因子 药物诱导作用或”效果” 各种报告基因的优点和缺点 萤光素酶报告基因的主要优点 非放射性 检测快（比CAT等 ... WebJun 14, 2024 · The supernatant was discarded to remove ATP derived from dead bacteria and SBA reagents. The remaining bacterial pellets were resuspended in PBS, transferred …
WebMar 16, 2024 · Item BacTiter-Glo Microbial Cell Viability Assay Company Promega Price Supplier Page View Company Product Page Catalog Number G8231 Target Microbial Cell Viability Quantity 10 x 10ml Detection luminescence Sample Type Live Cells Method Luminescent Promega 2800 Woods Hollow Rd. Madison, Wisconsin United States Phone: … WebHere, we describe the use of the BacTiter-Glo™ Microbial Cell Viability Assay to measure biofilm attachment. This 96-well-format assay monitors intracellular ATP concentrations. Since bacterial cells maintain a relatively constant intracellular ATP concentration, this assay provides a reproducible relative measure of attached cells.
WebThe CellTiter-Glo® 3D Cell Viability Assay is designed for determining cell viability in 3D microtissue spheroids. The assay reagent penetrates large spheroids and has increased lytic capacity—allowing more accurate determination of viability … WebThe BacTiter-Glo™ Microbial Cell Viability Assay is a homogeneous method to determine the number of viable bacterial cells in culture based on quantitation of ATP. The assay is designed for either single-tube or multiwell-plate formats for …
WebJun 5, 2012 · Promega 成功地开发出基于另一种萤火虫 Photinus pennsylvanica 的一种稳定形式的萤光素酶（Ultra-Glo TM 重组萤光素酶），使用一种能选 择在ATP 检测性能方面有所提高的性质。 另外，我们开发了一个专利配方，可以从不同 的微生物细胞中快速有效地提取ATP（表1）。 BacTiter-Glo TM 试剂中这两个基本因素的 结合使我们能够设计出在培养 …
WebThe antimicrobial activity was evaluated by means of the adenosine triphosphate (ATP) assay (BacTiter-Glo; Promega, Madison, WI, USA) and confocal laser scanning microscopy (CLSM). ... The luminescence produced was measured with a luminometer (GloMax; Promega, Madison, WI, USA), expressed as absolute values of relative light units ... having obtained help from god i continueWebThe Viral ToxGlo™ Assay is a simple, quantifiable method of determining viral-induced cytopathic effects (CPE) in host cells caused by lytic virions. The assay measures cellular ATP as a surrogate measure of host cell viability. When CPE occurs due to viral infection, ATP depletion can be measured and correlated with viral burden. having occult or metaphysical significanceWebJun 14, 2024 · The supernatant was discarded to remove ATP derived from dead bacteria and SBA reagents. The remaining bacterial pellets were resuspended in PBS, transferred to a white 96-well plate (Greiner Bio-One, Roma, Italy) and mixed 1:1 v: v with BacTiter-Glo Reagent (Promega, Southampton, UK). The reaction was incubated for 5 min at room … having occult or metaphysical nature or forceWebThe BacTiter-Glo Microbial Cell Viability Assay is a homogeneous method for determining the number of viable microbial cells in culture based on quantitation of the ATP present. A Luminescent Assay to Measure the Number of Viable Microbial Cells in Culture A homogeneous, single reagent assay Offers increased sensitivity, fast results bosch dishwasher bottom rack not cleanWebFeb 7, 2024 · After the incubation, the top chambers were removed and 230 μL of CellTiter-Glo (Promega, Madison, WA, USA) was added into each well according to the … having occult or metaphysicalWebBacTiter-Glo™ Microbial Cell Viability Assay: Bacterial cells in culture medium: Measures the number of viable bacterial cells. Designed for difficult-to-lyse bacterial samples. Can be used for single sample analysis or high-throughput applications. Very strong >30 minutes: Water-Glo™ Microbial Water Testing Kit: Water samples having occult nature or forceWebThe CellTiter-Glo® Assay is designed for use with multiwell formats, making it ideal for automated high-throughput screening (HTS), cell proliferation and cytotoxicity assays. The homogeneous assay procedure involves adding the single reagent (CellTiter-Glo® Reagent) directly to cells cultured in serum-supplemented medium. having ocd anxiety and depression