Organotypic hippocampal slices
WitrynaHere we summarize and review studies using hippocampal slice cultures to investigate mechanisms and treatment strategies for the neurodegenerative disorders like stroke (cerebral ischemia), Alzheimer's disease (AD) and epilepsia. Witryna6 kwi 2024 · Organotypic hippocampal slices were prepared from Wistar rats of both sexes (Jackson-No. 031628) at post-natal days 5–7 as previously described 59. In brief, dissected hippocampi were cut into ...
Organotypic hippocampal slices
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Witryna5 mar 2013 · In this study, we evaluated our simple method using the serum-free culture medium for successful adult organotypic hippocampal slice culture. Several tens of … WitrynaAn increasing body of data has shown that matrix metalloproteinase-9 (MMP-9), an extracellularly acting, Zn2+-dependent endopeptidase, is important not only for pathologies of the central nervous system but also for neuronal plasticity. Here, we use three independent experimental models to show that enzymatic activity of MMP-9 …
WitrynaOrganotypic hippocampal slices, an emerging tool to model synucleinopathies. Organotypic hippocampal slices, an emerging tool to model synucleinopathies … Witrynaorganotypic cultures of hippocampal slices to study potential mechanisms involved in iron-induced neuronal damage. Exposure of mature hippocampal slices to ferrous sulfate resulted in concentration- and time-dependent cell death. After iron treatment, markers of ROS formation and lipid peroxidation, i.e. intensity of dichlorofluorescein …
Witryna5 mar 2013 · In this sense, the organotypic hippocampal slice culture (OHSC), if generated from adult brain, would be a promising ex vivo tool for various kinds of … Witryna1 paź 2015 · In organotypic hippocampal slice cultures it was shown (Kreutz et al., 2011) that ganglioside GM1 exhibited a neuroprotective activity on beta-amyloid …
Witryna30 lip 2015 · Organotypic hippocampal slice cultures (OHSCs) have been used as a powerful ex vivo model for decades. They have been used successfully in studies of …
Witryna17 gru 2015 · Organotypic slices can be obtained from different brain regions (as described in Figure 1 ), but for the study of adult neurogenesis in normal and pathological conditions, the hippocampal region containing the SGZ and the slices containing the SVZ are most preferred. dewalt multi tool stone cutting bladeWitryna19 gru 2024 · Here we describe the use of an organotypic hippocampal slice model for studying α-synuclein aggregation and inter-neuronal spreading initiated by … church of christ sherman txWitryna18 sty 2024 · Organotypic hippocampal slice culture (OHSC) Rat organotypic hippocampal-entorhinal cortical slice cultures were prepared as described previously [48, 49].Briefly, neonates rat pups at postnatal day 7 (P7) were decapitated, brain removed and hippocampal-entorhinal complex dissected in Gey’s buffer (Sigma … dewalt multi tool uk best priceWitrynaMouse organotypic hippocampal slice cultures presented as a robust model for studying biochemical processes in developing brain, … church of christ shsWitryna23 kwi 2024 · Organotypic hippocampal slices to study α-syn aggregates spreading: Using of organotypic hippocampal slices offers a simple unidirectional circuit of … church of christ shooting txWitryna2 lis 2006 · The first studies on hippocampal organotypic slices aimed to assess their physiological significance using acute slices as comparison 1, 9, 10. They confirmed that synaptic as well as... dewalt n152704 hard storage caseWitryna20 cze 2013 · Improved Immunohistochemical Protocol for Organotypic Brain Slice Cultures. Overview. ... methods in order to obtain continuous multi-fluorescence labelling throughout the full thickness of cultured hippocampal slices whilst maintaining structural integrity. Slice cultures, processed as whole mounts for triple immunofluorescence … church of christ shooting full video