Lamp1 abcam ab24170
Tīmeklis2024. gada 13. apr. · We revealed that RIPK1 knockdown upregulated the protein levels of Hsp70.1B and increased the colocalization of Lamp1 and Hsp70.1B in ischemic astrocytes. ... ab2923, Abcam, UK) overnight at 4 °C ... TīmeklisThe antibodies used in this research were Tom20 (Abcam, ab186735) and LAMP1 (Abcam, ab24170). Followingly, these cells were incubated with the secondary antibodies (Anti-mouse IgG, CST, #4408 for Tom20 and Anti-rabbit IgG, CST, #8889 for LAMP1) for 2 hours in dark room.
Lamp1 abcam ab24170
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Tīmeklis(ab24170) Anti-LAMP1 antibody - Lysosome Marker Sign in or register to save this reagent to your favourites Supplier Abcam Host Rabbit Type Primary Clonality … TīmeklisThis can be assessed by (a) Western blot (WB) or immunofluorescence using anti-LAMP1 antibodies (Hybridoma bank, H4A3 and Abcam, ab24170) or (b) measuring lysosome compartment size by analysing LysoTracker fluorescence intensity (see Section 4.4.1). Furthermore, a complementary analysis of lysosomal activity should …
Tīmeklis779 publications reference this Antibody We have found 779 publications or academic papers that reference the product ab24170 (Anti-LAMP1 antibody - Lysosome Marker) supplied by Abcam so far. If you have written or know of another publication, please do let us know! Filter citations Tīmeklis2024. gada 3. febr. · For LAMP1 staining, sections were incubated with LAMP1 (Abcam, ab24170) diluted 1:200 for 2 hours at RT. The intensity of the staining along with its distribution (granular or diffuse) was noted. For the Ki67 staining performed on murine tumors, tissues were fixed in formalin and embedded in paraffin. Sections of …
Tīmeklis2024. gada 23. sept. · For immunofluorescence: rat anti-HA (3F10, Roche), rabbit anti-LAMP1 (ab24170, Abcam), donkey anti-rat AlexaFluor488 (Jackson Immunoresearch), donkey anti-rabbit AlexaFluor647 (Jackson ... Tīmeklis2014. gada 19. maijs · The primary antibodies include rabbit against EEA1 (Abcam ab2900), rabbit against Rab9 (Santa Cruz Biotechnology FL-201), rabbit against LAMP1 (Abcam ab24170), and rabbit against Giantin (Abcam ab24586). To measure the extent of colocalization between the fluorescence signals of SNAs and protein markers, the …
Tīmeklis2024. gada 10. apr. · Proper Citation: (Abcam Cat# ab24170, RRID:AB_775978) Target Antigen: LAMP1 Host Organism: rabbit Clonality: polyclonal Comments: Applications: IHC-P, WB Antibody Name: Anti-LAMP1 antibody - Lysosome Marker Description: This polyclonal targets LAMP1 Target Organism: human Antibody ID: AB_775978 …
TīmeklisSpecificity Murine CD107a/LAMP1 (Mr 110-140 kDa with a core protein of Mr ~40 kDa) Tested applications Suitable for: IHC-P, Flow Cyt (Intra) more details Species … looking at other people\u0027s calendar in outlookTīmeklisab24170 staining LAMP1 in human kidney tissue sections. Staining correlates with lysosomal specificity, particularly in the proximal … looking at our school 2022Tīmeklis2024. gada 23. febr. · After treatment, the cells were fixed with 4% paraformaldehyde for 30 min. Then, the cells or rehydrated brain paraffin slices were treated with Triton X-100 and blocked with BSA. The following primary antibodies were then added: rabbit polyclonal anti-mTOR (1:250; ab134903; Abcam, UK) and rabbit polyclonal anti … hops and hyzerTīmeklisCells were washed three times with PBS before fixing with paraformaldehyde and processed for immunofluorescence using LAMP1 (Abcam, Catalog No. ab24170) as a lysosomal marker and DAPI (ThermoFisher, Catalog No. 62248) as a DNA stain both at 1:1000 dilutions. Techniques: In Vivo, Imaging, Diffusion-based Assay, Injection hops and moreTīmeklisab25630 staining LAMP1 in human HaCaT keratinocytes by Immunocytochemistry/ Immunofluorescence. Cells were fixed with acetone, permeabilized with ice cold … hops and hounds san antonio txTīmeklisDetects a band of approximately 48 kDa (predicted molecular weight: 45 kDa). 1/20. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. looking at other girl meme generatorhops and honey