WebAug 9, 2024 · The five hump peaks in the OOS sample also eluted out at a similar retention time of 2BHT-QM. However, no measurable improvement of the hump peak shape in the OOS sample was achieved under the modified HPLC conditions. As shown in Fig. 5, a broad peak at the same retention time (~ 5 min) of 2BHT-QM peak was observed in the … WebJun 12, 2007 · I am analysing drug in capsule (gelatin capsule) and getting a huge hump before the parent peak. The method works fine for the drug substance. the hump is coming where the two impurities are supposed to be. My mobile phase A is 5/95/0.1:ACN/H2O/TFA, mobile phase B is 95/5/0/1:ACN/H2O/TFA. The Column is C18 and the gradient is Initial-- …
Diagnosing HPLC Chromatography Problems
WebPeak shape can be influenced by the injection solvent. It is better to dissolve the sample in initial mobile phase condition when possible. In this example, injection of samples in a strong solvent (acetonitrile) when a weak mobile phase (methanol/water) was used resulted in peak broadening. WebPurge pump at high flow rate (e.g., 5-10 mL/min.), prime system if necessary. (Prime each pump head separately.) If system has check valve, loosen valve to allow air to escape. If problem persists, flush system with 100% methanol or isopropanol. If problem still persists, contact system manufacturer. swimming pools uae
High Performance Liquid Chromatography - Chemistry LibreTexts
WebNov 17, 2024 · The problem is that a chromatogram shows negative peaks after positive peak exiting, working with HPLC RI detector. The standard solution and the sample contain carbohydrates. MP: 25% H2O and... WebSep 18, 2024 · Peak tailing occurs when the peak asymmetry factor (As) is greater than 1.2 — although peaks with As greater than 1.5 are acceptable for many assays. This is … WebI have got good peak in standard in Acetonitrile but got extra peak that are merging with the original peak when I ran sample. Samples are in Tris-HCl and were extracted by ethyl … swimming pools tupelo