WebPD-10 Desalting Columns contain Sephadex G-25 resin, which allows rapid group separation of high molecular weight substances from ... HiTrap Desalting1 100 × 5 mL 11-0003-29 HiPrep™ 26/10 Desalting 1 × 53 mL 17-5087-01 HiPrep 26/10 Desalting 4 × 53 mL 17-5087-02 1 Pack size available by special order 11. 12 WebConnecting the column Connect the HiTrap Desalting 5 mL column to the system (Fig. 5, on page 2). To avoid introducing air into the column, connect the column “drop to drop”. Step Action 1 Attach a column clamp to the column holder rail on the instrument. 2 Remove the column stoppers and mount the column on the union connector.
HisTrap HP, 1 ml and 5 ml - Auburn University
WebDesalting Column Uses. Desalting columns are used with both proteins and nucleic acids. They provide a fast, simple way to purify these biomolecules away from salts and small molecules. Common uses of … WebIn this case, Ni2+ ions can be removed by desalting on a HiTrap™ Desalting, a PD-10 Desalting Column, or HiPrep™ 26/10 Desalting, (see Table 4). Leakage of Ni2+ from Ni Sepharose High Performance is very low under all normal conditions, lower than for other IMAC media tested. For ff7 on cell phone
Instructions for Use - Cytiva
WebHisTrap™ FF crude Kit is designed for rapid and convenient manual purification of histidine-tagged proteins using premade buffers and a syringe. Histidine-tagged proteins can be purified directly from unclarified cell lysates. This saves time because the pretreatment of the sample is minimized. The kit contains three ready-to-use 1 mL ... WebWe recommend HiPrep™ 26/10 Desalting, HiTrap™ Desalting or PD-10 Desalting columns. 2 Operation First time use or after long term storage: RESOURCE Q is supplied in 20% ethanol and RESOURCE S is supplied in 20% ethanol, 0.2 M sodium acetate. 1 To remove ethanol, wash with 5 column volumes of distilled ff7 nibelheim safe code