WebNov 3, 2024 · FBW7α was co-localized with TPP1 in cultured HeLa cells, as evidenced by confocal microscopy (Figure S2 Q). Thus, our results indicate that the FBW7 C-terminal tail region in the WD40 domain binds to the CPD containing phosphorylated 354 S and 358 S residues in the S/T region of TPP1, mediating TPP1 multisite polyubiquitination at the … WebFeb 27, 2024 · Fbw7α through its WD domain interacts with Runx2 both in a heterologous (HEK293T cells) system as well as osteoblasts. GSK3β was also present in the same complex as determined by co ...
A Nucleolar Isoform of the Fbw7 Ubiquitin Ligase Regulates c-Myc …
WebMar 21, 2024 · FBXW7 (F-Box And WD Repeat Domain Containing 7) is a Protein Coding gene. Diseases associated with FBXW7 include Developmental Delay, Hypotonia, And … WebMar 9, 2015 · USP36 interacts with and deubiquitinates c-Myc in cells and in vitro, leading to the stabilization of c-Myc. This USP36 regulation of c-Myc occurs in the nucleolus. … greenwood accountancy teignmouth
FBW7 Polyclonal Antibody (A301-720A) - Thermo Fisher Scientific
WebJan 18, 2024 · FBW7α was immunoprecipitated with 5 μg/ml FBW7α antibody (Bethyl Laboratories Inc., A301-720A). Rabbit IgG at 5 μg/ml was used as a negative control. Immunoprecipitated complexes were washed 5 times in 1× ice-cold CLB. Eluted samples were run on 12 % Tris-HCl gel for 5–10 minutes at 150 volts. Gels were washed with … WebFeb 13, 2007 · Myc-tagged Fbw7α was co-expressed with Flag-tagged Fbw7α or various N-terminal truncation mutants as well as a point mutant of Fbw7α that no longer interacts with CPDs. Lysates were immunoprecipitated with Flag antibody and probed as indicated. ΔN refers to an N-terminal-less Fbw7 construct corresponding to the common region of Fbw7. WebFbw7α had a greater effect than Fbw7β (supplemental Figure 6). Nonetheless, the difference in the Fbw7β versus Fbw7α effect on PGC-1α degradation appears to be directly related to the cellular localization of the Fbw7 species. This is seen with Fbw7α, Fbw7β, and the chimeric protein mutants (Figures 4, ,5, 5, ,6, 6, and and7). 7 ... foamiran hobby lobby